The project

A portable MicroNanoBioSystem and Instrument for ultra-fast analysis of pathogens in food: Innovation from LOVE-FOOD lab prototype to a pre-commercial instrument.

LoveFood2Market

The development of new methodologies advancing the state of the art in foodborne pathogen detection is a challenge for scientists and technologists as well as food industry and consumers. This project aims to meet the challenge by providing a reliable and versatile solution thanks to the convergence of micro-nano-bio systems. The work capitalizes on several innovative concepts which have already been proven to meet the required criteria for fast, low cost and highly sensitive analysis of pathogens in food samples in a previous research project entitled LoveFood.

These concepts are gathered on a credit-card size Lab-on-Chip platform, where all necessary steps for bacteria detection are performed on several chips. Specifically, bacteria capture and lysis (one chip), DNA extraction (second chip) and amplification (third chip) and finally pathogenic-DNA detection (fourth chip) can be performed in less than 8 hours and without the need for skilled personnel or large, lab-based dedicated equipment.

To proceed for a higher Technology Readiness Level towards the successful commercialization of the current prototype and produce a portable, and rapid platform (targeting total pathogen analysis time less than 4 hours including a 3 hour pre-enrichment step), we propose to further develop it by integrating the bacteria lysis, DNA purification and amplification modules, as well as the biochip detection platform on a single cartridge, able to perform multi-pathogen analysis (i.e. Salmonella, Listeria, E.coli and B. cereus) in multiple samples. The system will be developed for dairy products and meat analysis, with a strong commitment to produce a pre-industrial prototype by the end of the project.

Partners

FORTH

Foundation for Research & Technology Hellas Greece

Prof. E. Gizeli, Institute of Molecular Biology & Biotechnology
www.imbb.forth.gr/personal_page/gizeli.html

PROJECT COORDINATOR

Demokritos

National Center for Scientific Research “Demokritos” Greece

Dr A. Tserepi and Dr E. Gogolides, Institute of Nanoscience and Nanotechnology (INN)
inn.demokritos.gr
Institute Pasteur

Institut Pasteur France

Dr. B. Dupuy

www.pasteur.fr/ip/easysite/pasteur/en
Jobst Technologies

Jobst Technologies Germany

Dipl. HTL Ing. G. Jobst
www.jobst-technologies.com
SENSeOR

SENSeOR France

Dr Fr. Gégot
www.senseor.com
Mevgal

MEVGAL S.A. Greece

Dr H. Georgakis
www.mevgal.gr