END-USERS › First protocol for Salmonella detection in milk

5h Salmonella detection in milk samples

Protocol and methodology developed within EU project LoveFood2Market
 
Introduction

LF2M methodology relies on the use of advanced micro-nano-bio technologies and components for the rapid and simple detection of Salmonella Typhimurium strain in milk.  It consists of 3 steps and an unprecedented time-to-analysis of 5 h, including the pre-enrichment step.

Materials needed per test
Instrumentation
Protocol*
Step 1: Pre-enrichment (4 h)
Step 2: Washing and cell-lysis (30 min)
Step 3: Amplification and detection (30 min)

*The protocol can be used for the detection of 1-10 Salmonella cells in 25ml of milk

 

Salmonella detection in milk samples

 

For more info, contact Prof. E. Gizeli: gizeli@imbb.forth.gr